Capto mmc impres compression factor11/24/2023 ![]() ![]() The compression factor is 1.05-1.15 for Capto adhere in XK columns (the. Capto MMC/Capto MMC ImpRes: high ionic strength solution (e.g., 2 M NaCl in buffer) and at the same time increase pH to > 9 (e.g., Tris buffer with 2 M NaCl) Regenerate the medium by washing until the column effluent shows stable conductivity and pH values. In this study, we demonstrated that when Capto MMC ImpRes chromatography is conducted under weak partitioning mode, high throughput, good yield, and effective byproduct removal are simultaneously achieved.īispecific antibody (bsAb) Capto MMC ImpRes Light chain (LC)-missing byproduct Mixed-mode chromatography Weak partitioning mode WuXiBody.Ĭopyright © 2021 Elsevier Inc. In comparison with Capto MMC, Capto MMC ImpRes has both a reduced bead size and. However, the dynamic binding capacity (DBC) of Capto MMC ImpRes is relatively low under the selected condition, making the process less desirable for large-scale manufacturing. The rigid matrix of Capto MMC ImpRes allows high flow velocities in MAb processes. We previously showed that Capto MMC ImpRes mixed-mode chromatography under bind-elute mode can effectively remove this LC-missing species. Capto MMC ImpRes is based on the established high-flow agarose matrix, which gives excellent pressure/flow properties. While for bsAbs based on other platforms removal of such byproduct can pose considerable challenge to the downstream team, in this case WuXiBody's unique design makes separation relatively straightforward. Capto MMC is designed to increase speed and throughput in capture and intermediate purification. For purifying a WuXiBody-based symmetric bsAb, we identified a LC-missing species as a major byproduct. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. HiScreen Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto MMC ImpRes weak cation exchange multimodal resin. Its key feature is the replacement of one parental antibody's CH1/CL region with the T cell receptor (TCR) constant domain, a design that promotes cognate heavy chain (HC)-light chain (LC) pairing. ![]() The strong ion exchange ligand groups of Capto SP ImpRes (left) and Capto Q ImpRes (right) are well established in large-scale purifications. Among them, half antibody and homodimer are found with high frequency. WuXiBody is a bispecific antibody (bsAb) platform developed by WuXi Biologics. Capto SP ImpRes and Capto Q ImpRes extend the well-established Capto platform to include high-resolution media for late intermediate purification and polishing. During recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing. ![]()
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